Gradus
VOL 3, NO 1 (2016): SPRING (APRIL)
EXPERIMENTS FOR DEVELOPING MARKERS FOR TOBAMOVIRUS-RESISTANCE IN PEPPER (CAPSICUM SPP.) USING NBS PROFILING APPROACH
Abstract
For generating molecular markers for Tobamovirus-resistance alleles in pepper, we tested the application of NBS-profiling procedure, which specifically produces markers in or near resistance genes. The NBS-profiling method was applied to controlled crosses, in which the donor plants with L1, L3 and L4 resistance alleles were crossed with sensitive C. annuum ‘Albaregia’. Individuals of F2 progenies containing the three different resistance alleles, were phenotypically characterized and used for further analysis. DNA of resistant and susceptible F2 plants was digested and ligated with adapter. In the PCR reaction one adapter primer and one degenerate NBS specific primer were used. The PCR products were analyzed on 6% polyacrylamid gels. Partially co-segregating bands with the resistant phenotype L3 were found.
Keywords
Keywords: Pepper (Capsicum annuum L.), Tobamovirus-resistance, NBS profiling, molecular marker,
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